Autor: |
ZHANG Huazhi, LI Xiaoshuang, GUO Xiaoye, ZHAO Mingchi |
Jazyk: |
čínština |
Rok vydání: |
2019 |
Předmět: |
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Zdroj: |
Zhongliu Fangzhi Yanjiu, Vol 46, Iss 5, Pp 401-405 (2019) |
Druh dokumentu: |
article |
ISSN: |
1000-8578 |
DOI: |
10.3971/j.issn.1000-8578.2019.18.1422 |
Popis: |
Objective To investigate the antitumor effect of apatinib on breast cancer cells in vitro and its mechanism. Methods CCK-8 assay was used to detect the inhibitory effect of apatinib on the proliferation of breast cancer cell line MDA-MB-231. Annexin V-FITC/PI apoptosis detection kit was used to detect the effect of apatinib on the apoptosis of MDA-MB-231 cells. The effect of apatinib on lactate production in MDA-MB-231 cells was detected by lactate content assay kit. The effect of apatinib on the extracellular acidification rate of MDA-MB-231 cells was detected by glycolysis stress test kit. Results Apatinib inhibited the proliferation of breast cancer MDA-MB-231 cells in vitro in a concentration-dependent manner (P < 0.05). The half maximal inhibitory concentration (IC50) of apatinib in MDA-MB-231 cells was 1.56μmol/L for 48h treatment. Apatinib also induced the apoptosis of MDA-MB-231 cells in a concentration-dependent manner (P < 0.05). Apatinib inhibited intracellular lactate content in MDA-MB-231 cells in a concentration-dependent manner (P < 0.05). Apatinib also decreased extracellular acidification rate of MDA-MB-231 cells, including glycolytic capacity and glycolytic reserve (P < 0.05). Conclusion Apatinib could induce the apoptosis of breast cancer cell line MDA-MB-231 by inhibiting aerobic glycolysis. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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