Vaginal carriage of Haemophilus influenzae in a non-pregnant reproductive-age population

Autor:	Meghana A Limaye, Sara Brubaker, Tara M Randis, Adam J Ratner
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	Haemophilus influenzae Perinatal pathogen Vertical transmission Neonatal infection Vaginal microbiome Microbiology QR1-502
Zdroj:	BMC Microbiology, Vol 23, Iss 1, Pp 1-7 (2023)
Druh dokumentu:	article
ISSN:	1471-2180
DOI:	10.1186/s12866-023-02885-y
Popis:	Abstract Background Haemophilus influenzae (Hi) is an emerging cause of early onset neonatal sepsis, but mechanisms of transmission are not well understood. We aimed to determine the prevalence of vaginal carriage of Hi in reproductive age women and to examine behavioral and demographic characteristics associated with its carriage. Methods We performed a secondary analysis of stored vaginal lavage specimens from a prospective cohort study of nonpregnant reproductive-age women. After extraction of bacterial genomic DNA, samples were tested for the presence of the gene encoding Haemophilus protein d (hpd) by quantitative real-time polymerase chain reaction (PCR) using validated primers and probe. PCR for the V3-V4 region of the 16 S rRNA gene (positive control) assessed sample quality. Samples with cycle threshold (CT) value
Databáze:	Directory of Open Access Journals
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