Determination of the DNA repair pathways utilised by acute lymphoblastic leukaemia cells following daunorubicin treatment

Autor:	Hussain Mubarak Al-Aamri, Helen R. Irving, Terri Meehan-Andrews, Christopher Bradley
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Daunorubicin DNA double strand breaks (DSBs) Homologous recombination pathway (HR) Non-homologous end joining (NHEJ) pathway Medicine Biology (General) QH301-705.5 Science (General) Q1-390
Zdroj:	BMC Research Notes, Vol 12, Iss 1, Pp 1-5 (2019)
Druh dokumentu:	article
ISSN:	1756-0500
DOI:	10.1186/s13104-019-4663-8
Popis:	Abstract Objective DNA double strand breaks (DNA-DSBs) are among the most lethal DNA lesions leading to genomic instability and repaired by either homologous recombination (HR) or the non-homologous end joining (NHEJ) mechanisms. The purpose of this study was to assess the importance and the level of activation of non-homologous end joining (NHEJ) and homologous recombination (HR) DNA repair pathways in three cell lines, CCRF-CEM and MOLT-4 derived from T lymphocytes and SUP-B15 derived from B lymphocytes following treatment with chemotherapy agent daunorubicin. Results The Gamma histone H2AX (γH2AX) assay was used assess the effects of DNA-PK inhibitor NU7026 and RAD51 inhibitor RI-2 on repair of DNA-DSB following treatment with daunorubicin. In all cell lines, the NHEJ DNA repair pathway appeared more rapid and efficient. MOLT-4 and CCFR-CEM cells utilised both NHEJ and HR pathways for DNA-DSB repair. Whereas, SUP-B15 cells utilised only NHEJ for DSB repair, suggestive of a deficiency in HR repair pathways.
Databáze:	Directory of Open Access Journals
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