Vibrio cholerae O1 Avirulent Strains - Producers of Protective O1 Antigen: Obtaining and Peculiarities
| Autor: | E. N. Strelnikova-Aab, L. F. Livanova, A. A. Goryaev, N. B. Cheldyshova, N. I. Smirnova |
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| Jazyk: | ruština |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Проблемы особо опасных инфекций, Vol 0, Iss 4(106), Pp 39-42 (2010) |
| Druh dokumentu: | article |
| ISSN: | 0370-1069 2658-719X |
| DOI: | 10.21055/0370-1069-2010-4(106)-39-42 |
| Popis: | Among 15 Vibrio cholerae El Tor O1 virulent strains of Inaba and Ogawa serovars grown in sterile river water, 6 strains were found to lose spontaneously the core region of CTXφ prophage including structural genes (ctxAB) of cholera toxin. It was determined that the obtained ctxA- mutants did not produce cholera toxin and did not cause specific choleragenic reaction in the intra-intestinally inoculated suckling rabbits suggesting that they belonged to the IIId pathogenicity group. Analysis of the population structure of two selected non-toxigenic strains M569A and 5/65A revealed clones with high level of O1 antigen of Inaba and Ogawa serovars production. The obtained avirulent strains can be used as producers of above-noted protective antigens both in production of chemical cholera vaccine and in constructing of diagnostic immunoenzyme test-systems. |
| Databáze: | Directory of Open Access Journals |
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