| Autor: |
Guendalina Marini, Brad Poland, Chris Leininger, Natalya Lukoyanova, Dan Spielbauer, Jennifer K. Barry, Dan Altier, Amy Lum, Eric Scolaro, Claudia Pérez Ortega, Nasser Yalpani, Gary Sandahl, Tim Mabry, Jeffrey Klever, Timothy Nowatzki, Jian-Zhou Zhao, Amit Sethi, Adane Kassa, Virginia Crane, Albert L. Lu, Mark E. Nelson, Narayanan Eswar, Maya Topf, Helen R. Saibil |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Nature Communications, Vol 14, Iss 1, Pp 1-11 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2041-1723 |
| DOI: |
10.1038/s41467-023-39891-7 |
| Popis: |
Abstract The broad adoption of transgenic crops has revolutionized agriculture. However, resistance to insecticidal proteins by agricultural pests poses a continuous challenge to maintaining crop productivity and new proteins are urgently needed to replace those utilized for existing transgenic traits. We identified an insecticidal membrane attack complex/perforin (MACPF) protein, Mpf2Ba1, with strong activity against the devastating coleopteran pest western corn rootworm (WCR) and a novel site of action. Using an integrative structural biology approach, we determined monomeric, pre-pore and pore structures, revealing changes between structural states at high resolution. We discovered an assembly inhibition mechanism, a molecular switch that activates pre-pore oligomerization upon gut fluid incubation and solved the highest resolution MACPF pore structure to-date. Our findings demonstrate not only the utility of Mpf2Ba1 in the development of biotechnology solutions for protecting maize from WCR to promote food security, but also uncover previously unknown mechanistic principles of bacterial MACPF assembly. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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