| Autor: |
Cristina Xufré, Tanía González, Lorna Leal, Charles M. Trubey, Jeffrey D. Lifson, José María Gatell, José Alcamí, Núria Climent, Felipe García, Sonsoles Sánchez-Palomino |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Frontiers in Virology, Vol 2 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2673-818X |
| DOI: |
10.3389/fviro.2022.869431 |
| Popis: |
We described a novel HIV autologous isolation method based in coculturing macrophages and CD4+T-cell-enriched fractions from peripheral blood collected from antiretroviral-treated (ART) HIV patients. This method allows the isolation of high viral titers of autologous viruses, over 1010HIV RNA copies/ml, and reduces the time required to produce necessary amounts for virus for use as antigens presented by monocyte-derived myeloid cells in HIV therapeutic vaccine approaches. By applying these high titer and autologous virus produced in the patient-derived cells, we intended to elicit a boost of the immunological system response in HIV therapeutic vaccines in clinical trials. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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