| Autor: |
Xiao-dong LIANG, Jie DENG, Rui-xian YE, Huan-yan LIU, Qi-ying XIONG, Ru-jun XUE, Hui-heng CHEN, Yu-mei LIU, Jing-yao LIANG, Xi-bao ZHANG |
| Jazyk: |
čínština |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Pifu-xingbing zhenliaoxue zazhi, Vol 26, Iss 2, Pp 67-72 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
1674-8468 |
| DOI: |
10.3969/j.issn.1674-8468.2019.02.002 |
| Popis: |
Objective: To investigate the effect of all-trans retinoic acid (ATRA) on Cx43 protein expression and GJIC function in IL-22-treated HaCaT cells, and whether it is related to the JNK pathway to further elaborate molecular mechanism of ATRA on GJIC function. Methods: The optimal pretreated time affected by ATRA on HaCaT cells was screened in IL-22 treatment group.Scrape-loading dye-transfer assay was applied to observe the variation of GJIC function.The expression value of Cx43, p-JNK, JNK in ATRA and/or IL-22 treatment group were detected by immunofluorescence. Results: ATRA caused time-dependent increasing of GJIC function in HaCaT cells. After treatment of ATRA on IL-22 induced HaCaT cells, Cx43 protein expression level increased, GJIC function up-regulated, and p-JNK expression had not significant change. Conclusions: ATRA inhibited the decrease of Cx43 expression by IL-22-mediated and down-regulation of GJIC, and this process was not related to the JNK pathway. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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