Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes

Autor:	S. V. Ryzhov, K. S. Yuryeva, K. V. Goremykin, Ye. V. Korotkaya, I. V. Saltykova, Yu. A. Yakovleva, Ye. S. Kulikov, O. S. Fyodorova, Ye. E. Kremer, N. S. Fattakhov, A. E. Sazonov
Jazyk:	English<br />Russian
Rok vydání:	2011
Předmět:	моноциты аденозин аденозиновые рецепторы паракринные факторы интерлейкин-6 интерлейкин-8 фактор роста эндотелия сосудов Medicine
Zdroj:	Бюллетень сибирской медицины, Vol 10, Iss 3, Pp 54-61 (2011)
Druh dokumentu:	article
ISSN:	1682-0363 1819-3684
DOI:	10.20538/1682-0363-2011-3-54-61
Popis:	The flow cytometry analysis of human peripheral blood monocytes separated by a two step density gradient centrifugation is reported. The expression of mRNA level of adenosine receptor (AdoR) subtypes (A1, A2A, A2B and A3) and interleukin 6 (IL-6), interleukin 8 (IL-8) and vascular endothelial growth factor (VEGF) in monocytes were determined using real-time PCR. We found considerable variation across individuals in mRNA expression levels of paracrine factors after the stimulation of adenosine receptors. Our findings suggests the role of adenosinergic system accounted for interindividual differences in monocyte activation.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/a2772ef9bab5410da37349c1c2cb575d Zobrazit plný text záznamu View record in DOAJ