| Autor: |
Cristian Iribarren, Nicolás Plaza, Sebastián Ramírez-Araya, Diliana Pérez-Reytor, Ítalo M. Urrutia, Elisabetta Suffredini, Teresa Vicenza, Soledad Ulloa, Jorge Fernández, Paola Navarrete, Victor Jaña, Leonardo Pavez, Talía del Pozo, Gino Corsini, Carmen Lopez-Joven, Katherine García |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Microorganisms, Vol 12, Iss 3, p 504 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2076-2607 |
| DOI: |
10.3390/microorganisms12030504 |
| Popis: |
In recent studies, emphasis has been placed on the zonula occludens toxin (Zot) from the non-toxigenic Vibrio parahaemolyticus strain PMC53.7 as an agent inducing alterations in the actin cytoskeleton of infected Caco-2 cells and which appears as a relevant virulence factor. Universal zot primers were designed by the alignment of different types of zot gene and identification of conserved sequences to investigate the presence in diverse environmental and clinical V. parahaemolyticus isolates, in co-occurrence with virulence factors, such a hemolysins and secretion systems. The study screened a total of 390 isolates from environmental sources from Chile and Italy and 95 Chilean clinical isolates. The results revealed that around 37.2% of Chilean environmental strains and 25.9% of Italian strains, and 24.2% of clinical isolates carried the zot gene. The Zot-C2 cluster was present in 71.4% of Chilean environmental strains but absent in clinical isolates, while the Zot-C4 cluster was identified in 28.6% of environmental and 100% of clinical isolates. Understanding the role of zot in V. parahaemolyticus virulence is crucial, especially considering the risk associated with consuming diverse isolates from bivalves and the co-occurrence with virulence factors such as TDH, TRH or T3SS2. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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