| Autor: |
Ahmed Abdal Dayem, Jihye Won, Hui-Gwan Goo, Gwang-Mo Yang, Dong Sik Seo, Byeong-Min Jeon, Hye Yeon Choi, Sang Eun Park, Kyung Min Lim, Seon-Ho Jang, Soo Bin Lee, Sang Baek Choi, Kyeongseok Kim, Geun-Ho Kang, Gyu-Bum Yeon, Dae-Sung Kim, Ssang-Goo Cho |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Stem Cell Research, Vol 43, Iss , Pp - (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1873-5061 |
| DOI: |
10.1016/j.scr.2020.101700 |
| Popis: |
Pluripotent stem cells (PSCs) offer a promising tool for regenerative medicine. The clinical application of PSCs inevitably requires a large-scale culture in a highly defined environment. The present study aimed to devise defined coating materials for the efficient adhesion and proliferation of human PSCs (hPSCs). We tested the activity of seven fibronectin-derived peptides and three laminin-derived peptides for the attachment and proliferation of hPSCs through their immobilization on the bottom of culture dishes by creating a fusion protein with the mussel adhesion protein. Among the extracellular matrix (ECM) mimetics tested, one fibronectin-derived peptide, PHSRN-GRGDSP, significantly promoted adhesion, enhanced alkaline phosphatase activity, and increased pluripotency-related gene expression in hPSCs compared to Matrigel. Furthermore, co-immobilization of a particular canofin peptide derived from fibroblast growth factor 2 increased pluripotency marker expression, which may offer the possibility of culture without growth factor supplementation. Our findings afford a novel defined condition for the efficient culture of hPSCs and may be utilized in future clinical applications. Keywords: Human induced pluripotent stem cell, Stem cell, Niche, ECM motif, Proliferation, Pluripotency, Adhesion |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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