Autor: |
Hao-Kai Li, Chi-Fon Chang, Hsuan-Ju Lin, Jung-Lee Lin, Yu-Ting Lee, Yu-Hsuan Wu, Chiao-Yen Liu, Tze-Chia Lin, Pang-Hung Hsu, Hong-Ting Victor Lin |
Jazyk: |
angličtina |
Rok vydání: |
2021 |
Předmět: |
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Zdroj: |
Fermentation, Vol 7, Iss 3, p 129 (2021) |
Druh dokumentu: |
article |
ISSN: |
2311-5637 |
DOI: |
10.3390/fermentation7030129 |
Popis: |
4-Mercapto-4-methyl-2-pentanone (4MMP), a high-impact aroma compound with the box tree and black currant flavors was first identified in wines and could be released by microbial cysteine-S-conjugate β-lyases from its precursors. In this study, various yeasts and bacteria encoding β-lyases were selected to examine their β-lyase activities. A thiol precursor of 4MMP, cysteine-conjugate of 4MMP (cys-4MMP), was synthesized with a purity of >95% in a relatively environmentally friendly approach, and its chemical structure was confirmed by nuclear magnetic resonance spectroscopy. The β-lyase activities of the crude cell extract from the bacteria and yeast strains for different substrates were examined using a colorimetric method. Shewanella putrefaciens cell extract exhibited the highest β-lyase activity for all tested substrates. Additionally, the optimum pH and temperature for their β-lyase activities were determined. To monitor the conversion efficiency of precursor cys-4MMP to 4MMP, liquid chromatography-mass spectrometry was used. Our data indicate that selected bacteria and yeasts could convert cys-4MMP into 4MMP, and S. putrefaciens exhibited the best conversion yield. This study demonstrated the potential use of microbial cell extracts to produce sulfur-containing aroma compounds such as 4MMP. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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