Determination of inosine 5’-monophosphate (IMP) and guanosine 5’-monophosphate (GMP) in yeast extracts using UV spectroscopy and chemometrics

Autor: Regina Andreia Ferrari Pereira, Cássia Ugucione, Edenir Rodrigues Pereira-Filho
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2018
Předmět:
Zdroj: Brazilian Journal of Food Technology, Vol 21, Iss 0 (2018)
Druh dokumentu: article
ISSN: 1981-6723
DOI: 10.1590/1981-6723.12717
Popis: Abstract This study proposed a method to determine the sum of the concentrations of inosine 5’-monophosphate (IMP) and guanosine 5’-monophosphate (GMP) in final and intermediate products through a combination of UV spectroscopy and multivariate calibration. Partial Least Squares regression (PLS regression) was used for the data analysis and the proposed methods presented errors of around 4 mg/L. In addition, the analysis cost is 10 times lower than the reference chromatographic method and the analytical frequency is 20 samples/hour.
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