| Autor: |
Janaina Coser, MSc, Thaís da Rocha Boeira, MSc, André Salvador Kazantzi Fonseca, MSc, Nilo Ikuta, PhD, Vagner Ricardo Lunge, PhD |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Brazilian Journal of Infectious Diseases, Vol 15, Iss 5, Pp 467-472 (2011) |
| Druh dokumentu: |
article |
| ISSN: |
1413-8670 |
| DOI: |
10.1016/S1413-8670(11)70229-X |
| Popis: |
Background: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. Objectives: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene. Methods: Analysis of published DNA sequence of mucosal HPV types to select sequences of new primers. Design of an original nested-PCR assay using the new primers pair selected and classical MY09/11 primers. HPV detection and typing in cervical samples using the nested-PCR-RFLP assay. Results: The nested-PCR-RFLP assay detected and typed HPV in cervical samples. Of the total of 128 clinical samples submitted to simple PCR and nested-PCR for detection of HPV, 37 (28.9%) were positive for the virus by both methods and 25 samples were positive only by nested-PCR (67.5% increase in detection rate compared with single PCR). All HPV positive samples were effectively typed by RFLP assay. Conclusion: The method of nested-PCR proved to be an effective diagnostic tool for HPV detection and typing. Keywords: DNA probes, HPV, polymerase chain reaction, polymorphism, restriction, fragment length |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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