Genome of the avirulent human-infective trypanosome--Trypanosoma rangeli.
| Autor: | Patrícia Hermes Stoco, Glauber Wagner, Carlos Talavera-Lopez, Alexandra Gerber, Arnaldo Zaha, Claudia Elizabeth Thompson, Daniella Castanheira Bartholomeu, Débora Denardin Lückemeyer, Diana Bahia, Elgion Loreto, Elisa Beatriz Prestes, Fábio Mitsuo Lima, Gabriela Rodrigues-Luiz, Gustavo Adolfo Vallejo, José Franco da Silveira Filho, Sérgio Schenkman, Karina Mariante Monteiro, Kevin Morris Tyler, Luiz Gonzaga Paula de Almeida, Mauro Freitas Ortiz, Miguel Angel Chiurillo, Milene Höehr de Moraes, Oberdan de Lima Cunha, Rondon Mendonça-Neto, Rosane Silva, Santuza Maria Ribeiro Teixeira, Silvane Maria Fonseca Murta, Thais Cristine Marques Sincero, Tiago Antonio de Oliveira Mendes, Turán Peter Urmenyi, Viviane Grazielle Silva, Wanderson Duarte DaRocha, Björn Andersson, Alvaro José Romanha, Mário Steindel, Ana Tereza Ribeiro de Vasconcelos, Edmundo Carlos Grisard |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | PLoS Neglected Tropical Diseases, Vol 8, Iss 9, p e3176 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1935-2727 1935-2735 |
| DOI: | 10.1371/journal.pntd.0003176 |
| Popis: | Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T. rangeli genome to provide new tools for elucidating the distinct and intriguing biology of this species and the key pathways related to interaction with its arthropod and mammalian hosts.The T. rangeli haploid genome is ∼ 24 Mb in length, and is the smallest and least repetitive trypanosomatid genome sequenced thus far. This parasite genome has shorter subtelomeric sequences compared to those of T. cruzi and T. brucei; displays intraspecific karyotype variability and lacks minichromosomes. Of the predicted 7,613 protein coding sequences, functional annotations could be determined for 2,415, while 5,043 are hypothetical proteins, some with evidence of protein expression. 7,101 genes (93%) are shared with other trypanosomatids that infect humans. An ortholog of the dcl2 gene involved in the T. brucei RNAi pathway was found in T. rangeli, but the RNAi machinery is non-functional since the other genes in this pathway are pseudogenized. T. rangeli is highly susceptible to oxidative stress, a phenotype that may be explained by a smaller number of anti-oxidant defense enzymes and heat-shock proteins.Phylogenetic comparison of nuclear and mitochondrial genes indicates that T. rangeli and T. cruzi are equidistant from T. brucei. In addition to revealing new aspects of trypanosome co-evolution within the vertebrate and invertebrate hosts, comparative genomic analysis with pathogenic trypanosomatids provides valuable new information that can be further explored with the aim of developing better diagnostic tools and/or therapeutic targets. |
| Databáze: | Directory of Open Access Journals |
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