| Autor: |
Kimberley R.G. Cortenbach, Daniel Morales Cano, Jelena Meek, Mark A.J. Gorris, Alexander H.J. Staal, Mangala Srinivas, I. Jolanda M. de Vries, Jacob Fog Bentzon, Roland R.J. van Kimmenade |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
International Journal of Cardiology: Heart & Vasculature, Vol 44, Iss , Pp 101111- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2352-9067 |
| DOI: |
10.1016/j.ijcha.2022.101111 |
| Popis: |
Background: Aim of this study was to investigate immune cells and subsets in different stages of human coronary artery disease with a novel multiplex immunohistochemistry (mIHC) technique. Methods: Human left anterior descending coronary artery specimens were analyzed: eccentric intimal thickening (N = 11), pathological intimal thickening (N = 10), fibroatheroma (N = 9), and fibrous plaque (N = 9). Eccentric intimal thickening was considered normal, and pathological intimal thickening, fibroatheroma, and fibrous plaque were considered diseased coronary arteries. Two mIHC panels, consisting of six and five primary antibodies, autofluoresence, and DAPI, were used to detect adaptive and innate immune cells. Via semi-automated analysis, (sub)types of immune cells in whole plaques and specific plaque regions were quantified. Results: Increased numbers of CD3+ T cells (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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