Effect of PAF on polyrnorphonuclear leucocyte plasma membrane polarity: a fluorescence study

Autor: A. Kantar, P. L. Giorgi, R. Fiorini
Jazyk: angličtina
Rok vydání: 1993
Předmět:
Zdroj: Mediators of Inflammation, Vol 2, Iss 2, Pp 149-151 (1993)
Druh dokumentu: article
ISSN: 0962-9351
1466-1861
09629351
DOI: 10.1155/S0962935193000225
Popis: The effect of PAF on the plasma membrane polarity of polymorphonuclear leukocytes (PMNs) was investigated by measuring the steady-state fluorescence emission spectra of 2-dimethylamino(6-1auroyl) naphthalene (Laurdan), which is known to be incorporated at the hydrophobic-hydrophilic interface of the bilayer, displaying spectral sensitivity to the polarity of its surrounding. Laurdan shows a marked steady-state emission blue-shift in non-polar solvents, with respect to polar solvents. Our results demonstrate that PAF (10−7 M) induces a blue shift of the fluorescence emission spectra of Laurdan. These changes are blocked in the presence of the PAF antagonist, L-659,989. Our data indicate that the interaction between PAF and PMNs is accompanied by a decrease in polarity in the hydrophobic-hydrophilic interface of the plasma membrane.
Databáze: Directory of Open Access Journals
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