Modern Immunotropic Therapy of Patients with Coronavirus Infections
| Autor: | O.K. Duda, L.P. Kotsyubaylo |
|---|---|
| Jazyk: | English<br />Ukrainian |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Aktualʹnaâ Infektologiâ, Vol 4, Iss 3.12, Pp 33-37 (2016) |
| Druh dokumentu: | article |
| ISSN: | 2312-413X 2312-4148 2312-413x |
| DOI: | 10.22141/2312-413x.3.12.2016.81709 |
| Popis: | Objective: to analyze the immune status of patients with coronavirus infections (CVI) and to improve the effectiveness of their treatment by including into the conventional scheme of a drug with antiviral and immunotropic action. Materials and methods. To achieve the objective, 150 patients aged 18 to 65 years with a diagnosis of acute respiratory moderate-to-severe and severe viral infection were examined during 2012–2015 at the infectious department of City Clinical Hospital № 4 of Kyiv. Subsequently, they were included in a group of patients with coronavirus infections (n = 36). The first (I, research one) group (n = 22) received ribonucleic acid 2 capsules 3 times a day, for 7 days, on the background of the main treatment. The second (II, control one) group (n = 14) underwent only conventional pathogenic therapy (detoxification and symptomatic). Verification of the diagnosis was carried out using molecular genetic studies (multiplex polymerase chain reaction with reverse transcription in real time) of the biological material — sputum. Complex of immunological studies of the peripheral blood included determining the receptors to monoclonal antibodies CD3, CD4, CD8, CD16, CD22 on blood lymphocytes, the content of T lymphocytes according to the data of spontaneous resetting ability of lymphocytes with sheep red blood cells by the method of M. Jondal et al. modified by K.F. Chernushenko et al. Statistical analysis of the findings was carried out with the aid of software system Windows, Word and Excel, Statistica 6.0, using the method of variation statistics with the definition of probability by means of the nonparametric Wilcoxon method for independent samples and Kruskal-Wallis analysis of variance. To compare two independent groups, we have used Mann-Whitney U-test. Results. An examination of 36 patients with coronavirus infections found that these diseases are accompanied by the development of secondary cellular immune deficiency with a decrease in major subpopulations of lymphocytes (CD3, CD4, CD22), indices of NBT-test of neutrophils and increased levels of CD16 lymphocytes. The total number of T lymphocytes and T helper cells was reduced (P |
| Databáze: | Directory of Open Access Journals |
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