Efficacy of garlic extract and chlorhexidine mouthwash in reduction of oral salivary microorganisms, an in vitro study

Autor: H T Ajay Rao, Sham S Bhat, Sundeep Hegde, Vikram Jhamb
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Ancient Science of Life, Vol 34, Iss 2, Pp 85-88 (2014)
Druh dokumentu: article
ISSN: 0257-7941
DOI: 10.4103/0257-7941.153465
Popis: Objectives: To assess and compare the antimicrobial effect of garlic extract and chlorhexidine (CHX) mouthwash solution against oral salivary microorganisms. Materials and Methods: Thirty six salivary samples were obtained in dry plastic vials. Collected saliva samples were centrifuged. Each vial was mixed completely on a shaker after which 1 ml of saliva was added to 9 ml of ethanol by a sterile pipette and mixed. A volume of 1 ml of garlic hydro-alcoholic extract and 1 ml of mouthwash was added to 1 ml each case specimen and was transferred to culture medium of Trypticase Soy Agar. Agar plates were incubated at 37°C for 48 h to allow for microbial growth. Microbial colonies were counted by independent interpreter to evaluate the result. Statistical Analysis: Data obtained were analyzed using one-way ANOVA test. P < 0.001 was considered statistically significant. Result: Result of the study shows that mean colony count of salivary microbial population was (1984 ± 400) 1127 in saline group (negative control), (50 ± 4) 27 in (0.12%) CHX group (positive control), (700 ± 200) 469 in garlic extract (5%) group (case control). Conclusion: Mouthwash containing garlic extract can be used as an alternative to CHX mouthwash.
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