Determinação simultânea de carbamazepina, fenitoína e fenobarbital em sangue seco em papel por cromatografia líquida de alta eficiência
| Autor: | Gabriela Martins Silva de Lima, Roberta Zilles Hahn, Cristina Rama, Liliane Rhoden, Paulina Hidalgo, Cleber Álvares da Silva, Marina Venzon Antunes, Rafael Linden |
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| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 37, Iss 6, Pp 1067-1071 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1678-7064 0100-4042 |
| DOI: | 10.5935/0100-4042.20140166 |
| Popis: | Carbamazepine, phenobarbital and phenytoin were determined in dried blood spots (DBS) by high performance liquid chromatography, after extraction of 8 mm DBS using a mixture of acetonitrile and methanol. Analytes were separated by reversed-phase chromatography, with a run time of 17 minutes. Intra-assay and inter-assay precisions were in the 5.3 to 8.4% and 3.3 to 5.2% ranges, respectively. Accuracy was in the 98.8 to 104.3% range. The method had sensitivity to detect all analytes at levels below minimum therapeutic concentrations. The analytes were stable at 4 ºC and room temperature for up to 12 days and at 45 ºC for 9 days. The method was applied to 14 paired clinical samples of blood serum and DBS. |
| Databáze: | Directory of Open Access Journals |
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