Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS
| Autor: | Sérgio Luiz Dalmora, Liberato Brum Junior, Ricardo Machado Ferretto, Paulo Renato de Oliveira, Thiago Barth, Maximiliano da Silva Sangoi |
|---|---|
| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 31, Iss 3, Pp 574-578 (2008) |
| Druh dokumentu: | article |
| ISSN: | 0100-4042 1678-7064 |
| DOI: | 10.1590/S0100-40422008000300021 |
| Popis: | A liquid chromatography-tandem mass spectrometry method with atmospheric pressure chemical ionization (LC-APCI/MS/MS) was validated for the determination of etoricoxib in human plasma using antipyrin as internal standard, followed by on-line solid-phase extraction. The method was performed on a Luna C18 column and the mobile phase consisted of acetonitrile:water (95:5, v/v)/ammonium acetate (pH 4.0; 10 mM), run at a flow rate of 0.6 mL/min. The method was linear in the range of 1-5000 ng/mL (r²>0.99). The lower limit of quantitation was 1 ng/mL. The recoveries were within 93.72-96.18%. Moreover, method validation demonstrated acceptable results for the precision, accuracy and stability studies. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|