Sphingosine-1-phosphate and ceramide are associated with health and atresia of bovine ovarian antral follicles
| Autor: | C.G. Hernández-Coronado, A. Guzmán, R. Espinosa-Cervantes, M.C. Romano, J.R. Verde-Calvo, A.M. Rosales-Torres |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Animal, Vol 9, Iss 2, Pp 308-312 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1751-7311 17517311 |
| DOI: | 10.1017/S1751731114002341 |
| Popis: | The follicle destiny towards ovulation or atresia is multi-factorial in nature and involves outcries, paracrine and endocrine factors that promote cell proliferation and survival (development) or unchain apoptosis as part of the atresia process. In several types of cells, sphingosine-1-phospate (S1P) promotes cellular proliferation and survival, whereas ceramide (CER) triggers cell death, and the S1P/CER ratio may determine the fate of the cell. The aim of present study was to quantify S1P and CER concentrations and their ratio in bovine antral follicles of 8 to 17 mm classified as healthy and atretic antral follicles. Follicles were dissected from cow ovaries collected from a local abattoir. The theca cell layer, the granulosa cells and follicular fluid were separated, and 17β-estradiol (E2) and progesterone (P4) concentrations were measured in the follicular fluid by radioimmunoassay. Based on the E2/P4 ratio, the follicles were classified as healthy (2.2±0.3) or atretic (0.2±0.3). In both follicular compartments (granulosa and theca cell layer), sphingolipids were extracted and S1P and CER concentrations were quantified by HPLC (XTerra RP18; 5 µm, 3.0×150 mm column). Results showed that in both follicular compartments, S1P concentrations were higher in healthy antral follicles than in atretic antral follicles (P |
| Databáze: | Directory of Open Access Journals |
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