Expression of CPAF, IFN-γ and IL-10 in different genotypes of Chlamydia trachomatis isolated from mice genital infection

Autor: Liu-yuan WANG, Pei-zhen ZHAO, Yao-hua XUE, Li-gang YANG, He-ping ZHENG, Bin YANG
Jazyk: čínština
Rok vydání: 2019
Předmět:
Zdroj: Pifu-xingbing zhenliaoxue zazhi, Vol 26, Iss 4, Pp 199-203 (2019)
Druh dokumentu: article
ISSN: 1674-8468
DOI: 10.3969/j.issn.1674-8468.2019.04.001
Popis: Objective: To analyze differences in expressions of CPAF, IFN-γ and IL-10 by establishing mouse genital tract infection with different CT genotypes. Methods: Seventy female Balb/c mice were randomly divided into E group, F group, J group, K group, H group, blank control group and experimental control group. Chlamydia trachomatis genotypes E, F, J, K and H (30 μL) were inoculated into vagina respectively. The blank control group was not treated, while the experimental control group was inoculated into vagina with 30 μL SPG. Expression of CPAF, IFN-γ and IL-10 in the uterus and fallopian tube was detected by ELISA on day 35 post-inoculation. Results: On day 35 post-inoculation, the concentration of CPAF in homogenate of uterus and fallopian tube in group H was significantly higher than that in group E, F and J (all P
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