Paprika Damping-off Outbreak Caused by Fusarium oxysporum Contaminated Seeds in Cheorwon Province in 2023
| Autor: | Miah Bae, Namsuk Kim, Sang Woo Kim, Sangyeon Ju, Byungyeon Kim, Soo Man Hwang, MeeKyoung Kim, Mi-Ri Park |
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| Jazyk: | English<br />Korean |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Research in Plant Disease, Vol 30, Iss 1, Pp 26-33 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1598-2262 2233-9191 |
| DOI: | 10.5423/RPD.2024.30.1.26 |
| Popis: | In 2023, symptoms like damping-off disease were observed in 74 paprika growing in greenhouses in Cheorwon-gun, Gangwon-do, Korea. In this study, we tried to find the cause of the damping-off disease outbreak. We collected symptomatic seedlings and observed the typical crescent-shaped conidia of Fusarium oxysporum by microscope. To confirm the presence of F. oxysporum in the samples, polymerase chain reaction was performed using primers specific for F. oxysporum; the resulting sequence showed 99.11% identity with F. oxysporum. To confirm the pathogenicity of the F. oxysporum (CW) isolated from the samples, healthy paprika plants were inoculated with F. oxysporum CW and damping-off symptoms were observed 2 weeks later. To investigate whether the damping-off disease outbreak in Cheorwon-gun was caused by F. oxysporum-contaminated seeds, 100 paprika seeds were disinfected and placed in Murashige and Skoog medium. Typical pink F. oxysporum hyphae were found only in control non-disinfected seeds. An 18S rRNA-based and a TEF gene-based phylogenetic analysis showed that the F. oxysporum CW isolate was not grouped with a F. oxysporum isolate reported from Cheorwon-gun in 2019. This study is the first report that an outbreak of damping-off disease in paprika in Cheorwon-gun, Gangwon-do, Korea, was caused by contamination of F. oxysporum seeds. |
| Databáze: | Directory of Open Access Journals |
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