Goat milk casein and protease sonication as a strategy to improve the proteolysis and functional properties of hydrolysates

Autor: Alécia Daila Barros Guimarães, Isabela Soares Magalhães, Alline Artigiani Lima Tribst, Eduardo Basílio de Oliveira, Bruno Ricardo de Castro Leite Júnior
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Food Chemistry Advances, Vol 3, Iss , Pp 100425- (2023)
Druh dokumentu: article
ISSN: 2772-753X
DOI: 10.1016/j.focha.2023.100425
Popis: This study investigated the impact of ultrasound (US) pretreatment on Alcalase and goat milk casein (GMC) to enhance GMC proteolysis. We assess the degree of hydrolysis (DH), TCA-soluble protein concentration, protein solubility and in vitro antioxidant activity. US pretreatment of Alcalase (40 °C/60 min) or GMC (60 °C/30 min) increased the relative enzyme activity by up to 15.6 and 18.1%, respectively. Hydrolysis using US pretreated samples increased the DH (up to 37%), and TCA-soluble protein concentration (up to 73%). This led to higher solubility (up to 37% at pH 4.0) and antioxidant activity (up to 37% in ABTS assay) of the hydrolysates after 180 min of reaction (p
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