Cytotoxic and Immunomodulatory Potential Activity of Fruit Extracts on Cervical Cancer (HeLa) and Fibroblast (L929) Cells
| Autor: | Helen Mier-Giraldo MSc, Luis Eduardo Díaz-Barrera MSc, PhD, Lucy Gabriela Delgado-Murcia PhD, Manuel Fernando Valero-Valdivieso PhD, Gabriela Cáez-Ramírez MSc, PhDc |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Journal of Evidence-Based Complementary & Alternative Medicine, Vol 22 (2017) |
| Druh dokumentu: | article |
| ISSN: | 2156-5872 2156-5899 21565872 |
| DOI: | 10.1177/2156587217718751 |
| Popis: | It was purposed to evaluate the biological potential of ethanol and isopropanol crude extracts of ripe Physalis peruviana fruits. Cytotoxic and immunomodulatory effects of the expression of interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 (MCP-1) were evaluated on human cervical cancer (HeLa) and murine fibroblast (L929) cells. The composition was evaluated by high-performance liquid chromatography diode-array detection and high-performance liquid chromatography ultraviolet/visible detection. The presence of ursolic acid and rosmarinic acid was found in both solvents. However, gallic acid, quercetin, and epicatechin were higher in isopropanol extracts ( P < .05). The results indicated a relationship among the total polyphenol content, antioxidant activity, and cytotoxic activity that was dependent on the solvent used. Isopropanol extracts presented a half-maximal inhibition concentration value (IC 50 ) of 60.48 ± 3.8 μg/mL for HeLa cells and 66.62 ± 2.67 μg/mL for L929 fibroblasts. The extracts reduced the release of interleukin-6, interleukin-8, and MCP-1 in a dose-dependent manner. Extracts showed anticancer and immunomodulatory potential for new complementary pharmaceutical products development. |
| Databáze: | Directory of Open Access Journals |
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