A leukocyte cryopreservation technique for cytogenetic studies
| Autor: | Duarte José Maurício Barbanti, Ramalho Mariney Flávia Pereira Di Tano, Lima Vera Fernanda Hossepian de, Jorge Wilham |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Genetics and Molecular Biology, Vol 22, Iss 3, Pp 399-400 (1999) |
| Druh dokumentu: | article |
| ISSN: | 1415-4757 1678-4685 |
| Popis: | Several culture, cryopreservation, freezing and thawing methods were tested to develop an efficient technique for storing cervid and ovine leukocytes. The best results were obtained with McCoy or Vega y Martinez (VYM) solution with dimethyl sulfoxide (DMSO), horse serum and polyvinylpyrrolidone (PVP) as cryopreservatives. The best protocol for freezing was 4°C for 30 min followed by 15 min in liquid nitrogen vapor. To thaw, the still frozen material was placed onto cultivation medium for propagation. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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