Highly efficient differentiation of embryonic stem cells into adipocytes by ascorbic acid
Autor: | Ixchelt Cuaranta-Monroy, Zoltan Simandi, Zsuzsanna Kolostyak, Quang-Minh Doan-Xuan, Szilard Poliska, Attila Horvath, Gergely Nagy, Zsolt Bacso, Laszlo Nagy |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: | |
Zdroj: | Stem Cell Research, Vol 13, Iss 1, Pp 88-97 (2014) |
Druh dokumentu: | article |
ISSN: | 1873-5061 1876-7753 |
DOI: | 10.1016/j.scr.2014.04.015 |
Popis: | Adipocyte differentiation and function have become the major research targets due to the increasing interest in obesity and related metabolic conditions. Although, late stages of adipogenesis have been extensively studied, the early phases remain poorly understood. Here we present that supplementing ascorbic acid (AsA) to the adipogenic differentiation cocktail enables the robust and efficient differentiation of mouse embryonic stem cells (mESCs) to mature adipocytes. Such ESC-derived adipocytes mimic the gene-expression profile of subcutaneous isolated adipocytes in vivo remarkably well, much closer than 3T3-L1 derived ones. Moreover, the differentiated cells are in a monolayer, allowing a broad range of genome-wide studies in early and late stages of adipocyte differentiation to be performed. |
Databáze: | Directory of Open Access Journals |
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