| Autor: |
Roschier Miia M, Mähönen Anssi J, Toivanen Pyry I, Määttä Antti I, Lesch Hanna P, Kärkkäinen Hanna-Riikka, Airenne Kari J, Laitinen Olli H, Ylä-Herttuala Seppo |
| Jazyk: |
angličtina |
| Rok vydání: |
2009 |
| Předmět: |
|
| Zdroj: |
BMC Research Notes, Vol 2, Iss 1, p 63 (2009) |
| Druh dokumentu: |
article |
| ISSN: |
1756-0500 |
| DOI: |
10.1186/1756-0500-2-63 |
| Popis: |
Abstract Background Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications. Findings We describe here protocols which adapt baculovirus generation into 96-well format. Conclusion The established methodology allows simple baculovirus generation, fast virus titering within 18 h and efficient recombinant protein production in a high-throughput format. Furthermore, the produced baculovirus vectors are compatible with gene expression in vertebrate cells in vitro and in vivo. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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