| Autor: |
Jiaqi Guan, Fei Liu, Sijia Zhao, Smith Etareri Evivie, Jialu Shi, Na Li, Li Zhao, Yingxue Yue, Qinggang Xie, Guicheng Huo, Bailiang Li |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Journal of Functional Foods, Vol 86, Iss , Pp 104715- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
1756-4646 |
| DOI: |
10.1016/j.jff.2021.104715 |
| Popis: |
Bifidobacterium species are the dominant bacteria in the intestinal tract of infants and young children, and are closely related to intestinal development. The Bifidobacterium longum subsp. longum strains K2-21-4 has been shown to promote the proliferation of CCD841 CoN cells. We evaluate the effect of B. longum on the proliferation of CCD841 CoN cells and tight junction using a lipopolysaccharide (LPS)-induced injury model. We discovered that B. longum K2-21-4 increased the cell proliferation rate and total cell protein content, shortened the cell cycle process, reduced apoptosis, and increased the activity of adenosine triphosphate (ATP)ases, aspartate aminotransferase, and alanine aminotransferase. Real-time reverse transcription quantitative polymerase chain reaction and western blotting showed that B. longum K2-21-4 activated Wnt/β-Catenin and PI3K/Akt/mTOR signaling pathway at gene transcription and protein expression levels up-regulated the expression level of protein related to the tight junction. Our findings suggest that B. longum K2-21-4 could regulate intestinal development by promoting cell proliferation and accelerating the maturation of the internal barrier. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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