Evaluation of Immune Responses Induced by Co-administration of Recombinant NP-based Influenza H1N1 Vaccine and Hemokinin-1 Adjuvant in BALB/c Mice
| Autor: | Mahdi Mehri, Farida Behzadian, Shahla Shahsavandi, Sina Soleimani |
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| Jazyk: | perština |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām, Vol 27, Iss 5, Pp 44-53 (2019) |
| Druh dokumentu: | article |
| ISSN: | 1563-4728 2588-3135 |
| Popis: | Introduction: The prevalence of influenza epidemics and direct transmission of the emerged avian virus strains to humans have highlighted the necessity of improving the management programs against this disease. The aim of this study was to evaluate the biological adjuvant effect of hemokinin-1 (HK-1) on enhancing the immune response induced by the recombinant influenza vaccine based on the Nucleoprotein protein (NP) of the H1N1 influenza virus. Materials & Methods: In this experimental study, a total of 40 six-week-old female BALB/c mice were divided into two control groups (5 mice per group) and three treatment groups (10 mice per group). The control groups received normal saline and HK-1 oligonucleotide adjuvant. On the other hand, the treatment groups received NP recombinant protein (twice), NP protein with HK-1 (once), as well as NP protein with HK-1 (twice). Based on the grouping, 0.1 ml of each sample was injected into the quadriceps femoris muscle of the mice. The treatment groups received a 1:10 (v/v) ratio of the NP protein and HK-1. The serum samples were collected prior to primary and reminder vaccinations in defined intervals. The immune responses were analyzed using enzyme-linked immunosorbent and lymphocyte proliferation assays. Ethics code: RSVRI.REC.98.010 Findings: The rise of the anti-influenza antibody in immunized mice with recombinant NP-based vaccine received HK-1 adjuvant was significant, compared to that in the control group (P |
| Databáze: | Directory of Open Access Journals |
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