Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed

Autor: Yuchen Zhang, Qingyu Wang, Yajing Liu, Shuo Dong, Yongqiang Zhang, Youcheng Zhu, Yu Tian, Jingwen Li, Zhuoyi Wang, Ying Wang, Fan Yan
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: International Journal of Molecular Sciences, Vol 24, Iss 18, p 14125 (2023)
Druh dokumentu: article
ISSN: 24181412
1422-0067
1661-6596
DOI: 10.3390/ijms241814125
Popis: Soybean (Glycine max (L.) Merr.) is an important source of plant protein, the nutritional quality of which is considerably affected by the content of the sulfur-containing amino acid, methionine (Met). To improve the quality of soybean protein and increase the Met content in seeds, soybean cystathionine γ-synthase 2 (GmCGS2), the first unique enzyme in Met biosynthesis, was overexpressed in the soybean cultivar “Jack”, producing three transgenic lines (OE3, OE4, and OE10). We detected a considerable increase in the content of free Met and other free amino acids in the developing seeds of the three transgenic lines at the 15th and 75th days after flowering (15D and 75D). In addition, transcriptome analysis showed that the expression of genes related to Met biosynthesis from the aspartate-family pathway and S-methyl Met cycle was promoted in developing green seeds of OE10. Ultimately, the accumulation of total amino acids and soluble proteins in transgenic mature seeds was promoted. Altogether, these results indicated that GmCGS2 plays an important role in Met biosynthesis, by providing a basis for improving the nutritional quality of soybean seeds.
Databáze: Directory of Open Access Journals
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