| Autor: |
Jaqueline Helena da Silva Santos, Andressa Coelho Sichi, Cintia Mayumi Ahagon, Norberto Camilo Campos, Luís Fernando de Macedo Brígido |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Journal of Clinical Virology Plus, Vol 2, Iss 4, Pp 100113- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2667-0380 |
| DOI: |
10.1016/j.jcvp.2022.100113 |
| Popis: |
Background: Improving the early identification of HIV-1-infected newborns with birth testing is critical to comprehensive early infant diagnosis and care for newborns living with HIV-1. Automated RNA quantification systems are valuable diagnostic tools, but the volume of plasma that viral load platforms require makes their widespread use for young children difficult. Method: Seventy-nine plasma samples with different viral load ranges were evaluated in parallel with the use of 1x PBS, pH 7.4, to supplement the required volume at dilutions factors from 1:2 to 1:50. Viral load quantification assays were evaluated using ABBOTT Molecular platforms, USA. Results: Using 1x PBS, at 1:10 dilution (70 µL plasma in 630 µL 1x PBS), a sensitivity of 100% and 100% specificity were obtained for detecting a viremia above 400 copies/mL (Kappa of 0.96, p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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