| Autor: |
Steven P. Proper, Alexander T. Dwyer, Andrews Appiagyei, Jennifer M. Felton, Netali Ben-Baruch Morgenstern, Justin M. Marlman, Michael Kotliar, Artem Barski, Ty D. Troutman, Marc E. Rothenberg, Tesfaye B. Mersha, Nurit P. Azouz |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Frontiers in Allergy, Vol 5 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2673-6101 |
| DOI: |
10.3389/falgy.2024.1323405 |
| Popis: |
IntroductionAtopic dermatitis (AD) is an allergic skin disease mediated by skin barrier impairment and IL-13-driven immune response. Activation of the aryl hydrocarbon receptor (AHR) has shown promise in early clinical trials for AD; however, the mechanism by which AHR partially ameliorates AD is not well known.MethodsGene expression data from human biopsies were analyzed, and compared to gene expression from RNA-sequencing in our in-vitro HaCaT cell model system. Western blot, ELISA qRT-PCR were used to further explore the relationship between AHR and IL-13 signaling in HaCaT cells.ResultsThe AHR target gene CYP1A1 was decreased in lesional skin compared with healthy control skin (p = 4.30 × 10−9). Single-cell RNA sequencing (scRNAseq) demonstrated increased AHR expression (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
