Autor: |
My Kieu Ha, Esther Bartholomeus, Luc Van Os, Julie Dandelooy, Julie Leysen, Olivier Aerts, Vasiliki Siozopoulou, Eline De Smet, Jan Gielen, Khadija Guerti, Michel De Maeseneer, Nele Herregods, Bouchra Lechkar, Ruth Wittoek, Elke Geens, Laura Claes, Mahmoud Zaqout, Wendy Dewals, Annelies Lemay, David Tuerlinckx, David Weynants, Koen Vanlede, Gerlant van Berlaer, Marc Raes, Helene Verhelst, Tine Boiy, Pierre Van Damme, Anna C. Jansen, Marije Meuwissen, Vito Sabato, Guy Van Camp, Arvid Suls, Jutte Van der Werff ten Bosch, Joke Dehoorne, Rik Joos, Kris Laukens, Pieter Meysman, Benson Ogunjimi |
Jazyk: |
angličtina |
Rok vydání: |
2022 |
Předmět: |
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Zdroj: |
Pediatric Rheumatology Online Journal, Vol 20, Iss 1, Pp 1-10 (2022) |
Druh dokumentu: |
article |
ISSN: |
1546-0096 |
DOI: |
10.1186/s12969-022-00747-x |
Popis: |
Abstract Background Transcriptome profiling of blood cells is an efficient tool to study the gene expression signatures of rheumatic diseases. This study aims to improve the early diagnosis of pediatric rheumatic diseases by investigating patients’ blood gene expression and applying machine learning on the transcriptome data to develop predictive models. Methods RNA sequencing was performed on whole blood collected from children with rheumatic diseases. Random Forest classification models were developed based on the transcriptome data of 48 rheumatic patients, 46 children with viral infection, and 35 controls to classify different disease groups. The performance of these classifiers was evaluated by leave-one-out cross-validation. Analyses of differentially expressed genes (DEG), gene ontology (GO), and interferon-stimulated gene (ISG) score were also conducted. Results Our first classifier could differentiate pediatric rheumatic patients from controls and infection cases with high area-under-the-curve (AUC) values (AUC = 0.8 ± 0.1 and 0.7 ± 0.1, respectively). Three other classifiers could distinguish chronic recurrent multifocal osteomyelitis (CRMO), juvenile idiopathic arthritis (JIA), and interferonopathies (IFN) from control and infection cases with AUC ≥ 0.8. DEG and GO analyses reveal that the pathophysiology of CRMO, IFN, and JIA involves innate immune responses including myeloid leukocyte and granulocyte activation, neutrophil activation and degranulation. IFN is specifically mediated by antibacterial and antifungal defense responses, CRMO by cellular response to cytokine, and JIA by cellular response to chemical stimulus. IFN patients particularly had the highest mean ISG score among all disease groups. Conclusion Our data show that blood transcriptomics combined with machine learning is a promising diagnostic tool for pediatric rheumatic diseases and may assist physicians in making data-driven and patient-specific decisions in clinical practice. |
Databáze: |
Directory of Open Access Journals |
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