Detection of respiratory pathogenic bacterial nucleic acid detection by Loop-mediated Isothermal Amplification in patients with bacterial pulmonary infections

Autor: Chuanzhu Wang, Ziyun Zhang, Xuedong Wang, Boke Zhang
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Practical Laboratory Medicine, Vol 37, Iss , Pp e00344- (2023)
Druh dokumentu: article
ISSN: 2352-5517
DOI: 10.1016/j.plabm.2023.e00344
Popis: Objective: Nucleic acid testing can accurately and rapidly identify the presence of pathogenic bacteria. In this study, we analyzed respiratory pathogenic bacteria nucleic acids by LAMP (Loop-mediated isothermal amplification) to clarify the clinical application in patients with bacterial pulmonary infections. Methods: Clinical data and specimens were collected from 99 patients with bacterial pulmonary infections from June 2021 to April 2023. We compared the differences between nucleic acid detection of LAMP and sputum culture. The correlation between inflammation manifestations of pulmonary imaging and the nucleic acid detection of LAMP was compared and analyzed. And the relationship between LAMP and blood inflammatory markers were analyzed. Results: The positive rate of LAMP using sputum specimens was significantly higher than that of sputum culture (P
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