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Objectives: Diabetes is associated with cardiovascular risk and increased arterial stiffness. Whether large artery stiffness is associated with diabetes per se or concomitant hypertension is currently unknown. Methods: Male, Wistar rats (6 weeks) were divided into control (n=8), control with anti-hypertensive treatment (telmisartan, 10mg/kg/day, n=8), induced diabetes (intraperitoneal streptozotocin, 50 mg/kg, confirmed by blood glucose measurement, n=12) and diabetes with anti-hypertensive treatment (n=12). At 18 weeks, rats were anaesthetised (urethane, 1.3 g/kg) and aortic pulse wave velocity (aPWV, aortic stiffness) measured invasively across a full range of physiological arterial pressure (intravenous phenylephrine, sodium nitroprusside, 30 μg/kg/min). Passive (elastin, collagen) and active (endothelial, smooth muscle function) components of stiffness were quantified using tensile testing and myography. Results: Conscious, systolic blood pressure was high in both control and diabetic animals (142±16, 132±22 mmHg) compared to control and diabetic animals on anti-hypertensive therapy (105±11, 119±14 mmHg, p |
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