Target-Guided Isolation and Purification of Antioxidants from Urtica laetevirens Maxim. by HSCCC Combined with Online DPPH-HPLC Analysis
| Autor: | Aijing Li, Mencuo La, Huichun Wang, Jianzhong Zhao, Yao Wang, Ruisha Mian, Fangfang He, Yuhan Wang, Tingqin Yang, Denglang Zou |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Molecules, Vol 28, Iss 21, p 7332 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1420-3049 03372286 |
| DOI: | 10.3390/molecules28217332 |
| Popis: | Urtica laetevirens Maxim. is used extensively in traditional Chinese medicine (TCM) for its potent antioxidative properties. In this study, three antioxidants were purified from U. laetevirens. using HSCCC guided by online DPPH-HPLC analysis. Firstly, the online DPPH-HPLC analysis was performed to profile out the antioxidant active molecules in U. laetevirens. The ultrasonic-assisted extraction conditions were optimized by response surface methodology and the results showed the targeted antioxidant active molecules could be well enriched under the optimized extraction conditions. Then, the antioxidant active molecules were separated by high-speed countercurrent chromatography ethyl acetate/n-butanol/water (2:3:5, v/v/v) as the solvent system. Finally, the three targets including 16.8 mg of Isovitexin, 9.8 mg of Isoorientin, and 26.7 mg of Apigenin-6,8-di-C-β-d-glucopyranoside were obtained from 100 mg of sample. Their structures were identified by 1H NMR spectroscopy. |
| Databáze: | Directory of Open Access Journals |
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