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Meng Chen,1,2 Yang Li,1,2 Wen-Xiu Hou,3 Da-Yong Peng,1,2 Jing-Kun Li,1,2 Hao-Xuan Zhang1,2 1Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong Province, 250014, People’s Republic of China; 2Department of Orthopedic Surgery, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong Province, 250014, People’s Republic of China; 3Department of Spine Surgery, Shandong University Qilu Hospital, Jinan, Shandong, 250000, People’s Republic of ChinaCorrespondence: Hao-Xuan Zhang, Department of Orthopedics, The First Affiliated Hospital of Shandong First Medical University, No. 16766, Lixia District, Jingshi Road, Jinan, Shandong Province, 250014, People’s Republic of China, Tel/Fax +86531-89268540, Email hoho0605@126.comPurpose: The repair and treatment of infected bone defects (IBD) is a common challenge faced by orthopedic clinics, medical materials science, and tissue engineering.Methods: Based on the treatment requirements of IBD, we utilized multidisciplinary knowledge from clinical medicine, medical materials science, and tissue engineering to construct a high-efficiency vancomycin sustained-release system with nanodiamond (ND) and prepare a composite scaffold. Its effect on IBD treatment was assessed from materials, cytology, bacteriology, and zoology perspectives.Results: The results demonstrated that the Van-ND-45S5 scaffold exhibited an excellent antibacterial effect, biocompatibility, and osteogenesis in vitro. Moreover, an efficient animal model of IBD was established, and a Van-ND-45S5 scaffold was implanted into the IBD. Radiographic and histological analyses and bone repair-related protein expression, confirmed that the Van-ND-45S5 scaffold had good biocompatibility and osteogenic and anti-infective activities in vivo.Conclusion: Collectively, our findings support that the Van-ND-45S5 scaffold is a promising new material and approach for treating IBD with good antibacterial effects, biocompatibility, and osteogenesis.Graphical Abstract: Keywords: antibacterial effect, nanodiamond, scaffold, infected bone defects |
