Autor: |
Pulak Maitra, Jubair Al-Rashid, Dipa Mandal, Md. Shofiul Azam, Noorain Munim Rasul |
Jazyk: |
angličtina |
Rok vydání: |
2021 |
Předmět: |
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Zdroj: |
Agronomy, Vol 11, Iss 5, p 1009 (2021) |
Druh dokumentu: |
article |
ISSN: |
2073-4395 |
DOI: |
10.3390/agronomy11051009 |
Popis: |
Nontoxic polymers PVP and Na-alginate may provide a favorable environment for the survival of bacteria. Therefore, PVP and Na-alginate were added to a growth medium to develop a liquid inoculant of Bradyrhizobium japonicum strain. The strain was identified by 16S rDNA sequencing. The addition of PVP (1.8%) and Na-alginate (0.2%) in the medium promoted a better survival (1.93 × 109 cells mL−1) of B. japonicum strain compared to the control (3.50 × 102 cells mL−1) after 6 months of storage. The combination of PVP and Na-alginate ensured 1.53 × 107 cells mL−1 up to 12 months of storage under ambient temperature (28 ± 2 °C), whereas PVP (1.8%) or Na-alginate (0.2%) alone produced similar cell counts only up to 8 months and 6 months, respectively. Consecutive field experiments proved the efficacy of the liquid inoculant on nodulation and yield of soybean. The combination of PVP and Na-alginate-based inoculation of B. japonicum strain significantly increased the nodule number per plant, number of pods per plant, number of seeds per pod, seed yield, and yield per hectare (p ≤ 0.05). Thus, the combination of PVP- and Na-alginate-based inoculation of B. japonicum has great potential to popularize the organic cultivation of soybean. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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