The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM

Autor: Edvin J. Thanikkal, Dharmender K. Gahlot, Junfa Liu, Marcus Fredriksson Sundbom, Jyoti M. Gurung, Kristina Ruuth, Monika K. Francis, Ikenna R. Obi, Karl M. Thompson, Shiyun Chen, Petra Dersch, Matthew S. Francis
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Virulence, Vol 10, Iss 1, Pp 37-57 (2019)
Druh dokumentu: article
ISSN: 2150-5594
2150-5608
21505594
DOI: 10.1080/21505594.2018.1556151
Popis: The Gram-negative enteropathogen Yersinia pseudotuberculosis possesses a number of regulatory systems that detect cell envelope damage caused by noxious extracytoplasmic stresses. The CpxA sensor kinase and CpxR response regulator two-component regulatory system is one such pathway. Active Cpx signalling upregulates various factors designed to repair and restore cell envelope integrity. Concomitantly, this pathway also down-regulates key determinants of virulence. In Yersinia, cpxA deletion accumulates high levels of phosphorylated CpxR (CpxR~P). Accumulated CpxR~P directly repressed rovA expression and this limited expression of virulence-associated processes. A second transcriptional regulator, RovM, also negatively regulates rovA expression in response to nutrient stress. Hence, this study aimed to determine if CpxR~P can influence rovA expression through control of RovM levels. We determined that the active CpxR~P isoform bound to the promoter of rovM and directly induced its expression, which naturally associated with a concurrent reduction in rovA expression. Site-directed mutagenesis of the CpxR~P binding sequence in the rovM promoter region desensitised rovM expression to CpxR~P. These data suggest that accumulated CpxR~P inversely manipulates the levels of two global transcriptional regulators, RovA and RovM, and this would be expected to have considerable influence on Yersinia pathophysiology and metabolism.
Databáze: Directory of Open Access Journals
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