| Autor: |
Zheng-Hui Zhao, Chun-Yang Li, Tie-Gang Meng, Yan Wang, Wen-Bo Liu, Ang Li, Yi-Jun Cai, Yi Hou, Heide Schatten, Zhen-Bo Wang, Qing-Yuan Sun, Qiang Sun |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Cell Discovery, Vol 6, Iss 1, Pp 1-16 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2056-5968 |
| DOI: |
10.1038/s41421-020-00219-0 |
| Popis: |
Abstract Germ cells are vital for reproduction and heredity. However, the mechanisms underlying female germ cell development in primates, especially in late embryonic stages, remain elusive. Here, we performed single-cell RNA sequencing of 12,471 cells from whole fetal ovaries, and explored the communications between germ cells and niche cells. We depicted the two waves of oogenesis at single-cell resolution and demonstrated that progenitor theca cells exhibit similar characteristics to Leydig cells in fetal monkey ovaries. Notably, we found that ZGLP1 displays differentially expressed patterns between mouse and monkey, which is not overlapped with NANOG in monkey germ cells, suggesting its role in meiosis entry but not in activating oogenic program in primates. Furthermore, the majority of germ cell clusters that sharply express PRDM9 and SPO11 might undergo apoptosis after cyst breakdown, leading to germ cell attrition. Overall, our work provides new insights into the molecular and cellular basis of primate fetal ovary development at single-cell resolution. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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