| Autor: |
Muersha Wusiman, Fariborz Taghipour |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Microsystems & Nanoengineering, Vol 10, Iss 1, Pp 1-13 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2055-7434 |
| DOI: |
10.1038/s41378-024-00803-4 |
| Popis: |
Abstract Lactate measurements provide an opportunity to conveniently evaluate bodily functions and sports performance. A molecularly imprinted fluorescence biochip provides an innovative way to achieve lactate measurement and overcomes the limitations of enzyme-based sensors. To realize this goal, ZnO quantum dots (QDs), a biocompatible sensing material, were combined with selective receptors comprised of molecularly imprinted polymers (MIPs). The lactate-selective imprinted polymers were formed using 3-aminopropyltriethoxysilane (APTES) and 5-indolyl boronic acid monomers. Furthermore, a new solid-phase sensing platform that overcomes the limitations of liquid-based sensors was developed to detect lactate in real-time. The platform consists of the biosensor chip with a thin-film sensing layer, an ultraviolet (UV) excitation source, and a portable light detector. The final sensor has a sensitivity of 0.0217 mmol L-1 for 0–30 mmol L-1 of lactate in phosphate-buffered saline (PBS) with a correlation coefficient of 0.97. The high sensor sensitivity and selectivity demonstrates the applicability of the ZnO QDs and synthetic receptors for sweat analysis. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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