СHANGES OF Glut1, mTOR AND AMPK1α GENE EXPRESSION IN PANCREATIC LYMPH NODE LYMPHOCYTES OF RATS WITH EXPERIMENTAL DIABETES MELLITUS
| Autor: | D. A. Putilin, A. M. Kamyshnyi |
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| Jazyk: | ruština |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Медицинская иммунология, Vol 18, Iss 4, Pp 339-346 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1563-0625 2313-741X |
| DOI: | 10.15789/1563-0625-2016-4-339-346 |
| Popis: | With the help of molecular genetic method we have investigated the level of mRNA gene expressions Glut1, mTOR and AMPK1α in PLN in pancreatic lymph nodes (PLN) of rats with streptozotocininduced diabetes mellitus (SIDM) and after administration of metformin. The levels of Glut1, mTOR and AMPK1α mRNA were determined by means of RT-PCR using CFX96™ thermocycler (Real-Time PCR Detection Systems, Bio-Rad, USA). Relative gene expression levels were calculated as ratios to GAPDH reference gene using ΔΔCt method. Statistical analysis was performed with available “Bio-Rad СFX Manager 3.1” software (Bio-Rad, USA). The mTOR+ positive lymphocytes were identified by means of monoclonal antibodies, using an indirect immunofluorescence method. Hyperglycemia was accompanied by transcriptional induction of glucose transporter Glut1 gene (9.9 to 28.9-fold, p |
| Databáze: | Directory of Open Access Journals |
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