Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times
| Autor: | A. S. Amal, R. I. Arifiantini, M. A. Setiadi, S. Said |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Journal of the Indonesian Tropical Animal Agriculture, Vol 44, Iss 2, Pp 135-145 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2087-8273 2460-6278 28568753 |
| DOI: | 10.14710/jitaa.44.2.135-145 |
| Popis: | The objectives of the present study were to compare and determine the best post-thawed characteristics of balinese bull sperm cryopreserved in three different extenders; animal based (Tris-clarified egg yolk (Tris-cEY)), and non-animal based extenders (Bioxcell® (lecithin based) and Optixcell® (liposome based)) in combination with three different equilibration times (30 minutes, 2 hours, 4hours). Thirty six ejaculates were collected from six Balinese bulls and frozen in three extenders (Tris-cEY, Bioxcell® and Optixcell®) after equilibration in three different times (30 minutes, 2hours and 4hours). Computer-assisted sperm analysis (CASA), hypo-osmotic swelling test (HOST) and eosin nigrosin staining were used in the post-thawed semen analysis. There was a significant interaction between equilibration time and extender type for sperm motility, viability and membrane integrity. Thirty minutes equilibration time had the lowest values (P0.05). Moreover, post-thawed semen which were extended in Optixcell® for 2 hours equilibration showed a better motility compared with the other extenders (P |
| Databáze: | Directory of Open Access Journals |
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