DEVELOPMENT OF THE TECHNIQUE FOR IDENTIFYING Z-PREPARATIONS IN BIOLOGICAL OBJECTS BY THE METHOD OF UV-SPECTROPHOTOMETRY
| Autor: | G. P. Chepurnaya, V. A. Kartashov, L. V. Chernova |
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| Jazyk: | ruština |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Разработка и регистрация лекарственных средств, Vol 0, Iss 3, Pp 160-169 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2305-2066 2658-5049 |
| Popis: | In the given article, the technique is described and the experimental data for identifying Zaleplon, Zolpidem and Zopiclone (Z-preparations) by method of UV - spectrophotometry isolated from liver tissue are determined. The isolation of Z-preparations was carried out with one of amphiphilic solvents - acetone; purification of impurities - by H-hexane extraction from an acidic medium; TLC was used for additional purification. The Z-preparations were identified by the sequential application of TLC screening and UV-spectrophotometry. The UV-spectrophotometric method was used to determine the quantitative content of the analysis substances. The parameters and validation characteristics of the method were determined, both in the study of native Z-preparations and those isolated from biological objects (linearity, specificity, robustness, and detection limits). |
| Databáze: | Directory of Open Access Journals |
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