Ultrasensitive assay of HER1, HER2, and heregulin-α in whole blood
| Autor: | Raluca-Ioana Stefan-van Staden, Oana-Raluca Musat, Damaris-Cristina Gheorghe, Ruxandra-Maria Ilie-Mihai, Catalina Cioates Negut, Paula Sfirloaga |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Talanta Open, Vol 6, Iss , Pp 100151- (2022) |
| Druh dokumentu: | article |
| ISSN: | 2666-8319 23825340 |
| DOI: | 10.1016/j.talo.2022.100151 |
| Popis: | Microsensors based on the physical immobilization of oleamides (N-(pyridine-3-yl-methyl) oleamide, and N-(2-mercapto-1H-benzo[d]imidazole-5-yl) oleamide) on paste containing nanographene decorated with spheroidal Cu were proposed for the molecular recognition and ultrasensitive simultaneous determination of HER1, HER2, and heregulin-α in whole blood. While for the assay of HER1 and HER2 there were not significant differences in the sensitivities recorded when N-(pyridine-3-yl-methyl) oleamide, and N-(2-mercapto-1H-benzo[d]imidazole-5-yl) oleamide were used to modify the nanographene paste, for the assay of heregulin-α, a higher sensitivity was recorded when N-(2-mercapto-1H-benzo[d]imidazole-5-yl) oleamide was used to modify the paste. Wide working concentration ranges were recorded for HER1 (between 10−13 and 10−8 mol L − 1), HER2 (between 10−12–10−10 mol L − 1), and heregulin-α (10−13–10−11mol L − 1). The student t-test showed that there is no significant difference between the results obtained by utilizing the two microsensors for screening tests of whole blood, at 99% confidence level. |
| Databáze: | Directory of Open Access Journals |
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