Cell therapy in the treatment of bronchiolitis obliterans in a murine model
| Autor: | Julio de Oliveira Espinel, Carolina Uribe, Fabíola Schons Meyer, Rafael Bringheti, Jane Ulbricht Kulczynski, Maurício Guidi Saueressig |
|---|---|
| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Předmět: | |
| Zdroj: | Revista do Colégio Brasileiro de Cirurgiões, Vol 42, Iss 3, Pp 181-188 |
| Druh dokumentu: | article |
| ISSN: | 1809-4546 0100-6991 |
| DOI: | 10.1590/0100-69912015003010 |
| Popis: | OBJECTIVE: To evaluate the importance of stem cells derived from adipose tissue in reducing graft inflammation in a murine model of allogeneic heterotopic tracheal transplant.METHODS: We performed a heterotopic tracheal allografting in dorsal subcutaneous pouch and systemically injected 5x105 mesenchymal stem cells derived from adipose tissue. The animals were divided into two groups according to the time of sacrifice: T7 and T21. We also carried out histological analysis and digital morphometry.RESULTS: The T7 animals treated with cell therapy had median obstructed graft area of 0 versus 0.54 of controls (p = 0.635). The treated T21 subjects had median obstructed graft area of 0.25 versus 0 in controls (p = 0.041).CONCLUSION: The systemically injected cell therapy in experimental murine model of bronchiolitis obliterans did not reduce the severity of the allograft inflammation in a statistically significant way in seven days; Conversely, in 21 days, it increased the allograft inflammatory process. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|