Potential mechanism of Achyranthis bidentatae radix plus semen vaccariae granules in the treatment of diabetes mellitus-induced erectile dysfunction in rats utilizing combined experimental model and network pharmacology

Autor: Ji-Sheng Wang, Jun-Long Feng, Heng-Heng Dai, Zi-Long Chen, Xiao Li, Bing-Hao Bao, Sheng Deng, Fan-Chao Meng, Qi Zhao, Hong-Sheng Xu, Bin Wang, Hai-Song Li
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Pharmaceutical Biology, Vol 59, Iss 1, Pp 547-556 (2021)
Druh dokumentu: article
ISSN: 1388-0209
1744-5116
13880209
35047364
DOI: 10.1080/13880209.2021.1920621
Popis: Context Achyranthes bidentata Blume (Amaranthaceae) (ABR) and semen vaccariae (SV) are used commonly in the clinical treatment of erectile dysfunction in males with diabetes mellitus (DMED) to strengthen the kidney and promote blood circulation, and often achieve good curative effects. Objective Explore mechanistic details of ABR + SV treatment against DMED. Materials and methods Prediction of key targets by network pharmacology. A rat model of DM was established by streptozotocin injection (55 mg/kg). Apomorphine (100 μg/kg) was injected into rats to screen the DMED model. Group C (n = 6) and group M (n = 6) were gavaged with deionized water; group T (n = 6) was given Achyranthis bidentatae radix–semen vaccariae granule suspension (2.5 g/kg). It lasted 8 weeks. Real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting (WB) were used to measure the expression of tissue-related proteins and mRNA. Results The predicted key targets are albumin (ALB), caspase-3 (CASP3), vascular endothelial growth factor A (VEGFA), angiotensin-converting enzyme (ACE), and endothelial nitric oxide synthase (eNOS). Compared with the M group (0.52 ± 0.04; 0.50 ± 0.03; 0.49 ± 0.02; 0.23 ± 0.03), CASP3, VEGFA, and ACE protein expression reduced in the T group (0.39 ± 0.06; 0.34 ± 0.03; 0.39 ± 0.03), and eNOS protein expression increased (0.34 ± 0.03). Conclusion ABR + SV can improve erectile function in DMED rats. This study provides a potential mechanism for the treatment of DMED with ABR + SV and can benefit from more patients.
Databáze: Directory of Open Access Journals
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