Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice
| Autor: | Yi Zhang, Ding-Ying Liao, Jian-Ming Wang, Li-Jun Wang, Xi-Ting Yang, Ai-Yi Zhou |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | International Journal of Ophthalmology, Vol 15, Iss 2, Pp 189-196 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2222-3959 2227-4898 44308051 |
| DOI: | 10.18240/ijo.2022.02.01 |
| Popis: | AIM: To investigate whether anti-placental growth factor (PGF) can inhibit subretinal fibrosis and whether this effect is mediated by the inhibitory effect of PGF on epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells. METHODS: Subretinal fibrosis model was established in laser induced choroidal neovascularization (CNV) mice on day 21 after laser photocoagulation. Immunofluorescence staining (IFS) of cryosections and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of PGF. IFS of whole choroidal flat-mounts was used to detect the degree of subretinal fibrosis. IFS of cryosections and ELISA were used to detect the expression of EMT related indicators in subretinal fibrosis lesions. RESULTS: The expression of PGF protein in subretinal fibrosis lesions was significantly up-regulated (P |
| Databáze: | Directory of Open Access Journals |
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