FT-IR spectroscopic characteristics of differently cultivated Escherichia coli
| Autor: | Zdenek FILIP, SUSANNE HERMANN, KATEŘINA DEMNEROVÁ |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Czech Journal of Food Sciences, Vol 26, Iss 6, Pp 458-463 (2008) |
| Druh dokumentu: | article |
| ISSN: | 1212-1800 1805-9317 |
| DOI: | 10.17221/14/2008-CJFS |
| Popis: | FT-IR spectra were recorded of Escherichia coli cell mass with the aim of obtaining spectral traits possibly useful in a rapid detection and characterisation of this indicator bacterium. A well differentiated spectrum was obtained from the cell mass harvested in a stationary phase of growth, e.g., after 24 h, from a minimum nutrient broth. The cell mass, harvested either earlier or grown in nutrient solutions which contained an enhanced carbon or nitrogen concentrations delivered somewhat different IR spectra, apparently due to a higher content of nucleic acid components as related to other structural constituents of bacterial cells. Consequently, the FT-IR spectra of E. coli, although rather rapidly to collect, seem only capable of delivering useful and reproducible information if the cell mass is obtained under standardised cultural conditions. |
| Databáze: | Directory of Open Access Journals |
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